Inhibitory Activity of Ethanol Extract of Green Betel Leaves (Piper betle Linn.) Using The UAE Method Against Trichophyton rubrum

Abstract

Abstract

Prevalence of dermatophytosis in Indonesia reaches 52%. Most cases are caused by the fungus Trichophyton rubrum. Side effects and resistance can be caused by antifungal drugs if used long-term, alternative treatment of plants like green betel leaves is needed. The aims of this study is to determine the anti-fungal effectiveness of green betel leaves ethanol extract with UAE method against Trichophyton rubrum. This research was conducted by means of experiments using concentrations of 10%, 20%, 30%, and 40%. The research data was obtained by measuring the inhibition zone for 2x24 hours using the disc method. The results of the 24-hours incubation showed that all concentrations were significant except concentrations of 30% to 40%. The results of the study on 48-hours incubation showed that all concentrations were not significantly different except concentrations of 10% to 30% and 40% and concentrations of 20% to 40% were meaningful. The results of the inhibition zone obtained are different because concentration and incubation time. Green betel leaves extract can inhibit Trichophyton rubrum because the active compounds contained, such as alkaloids, flavonoids, tannins, triterpenoids, and polyphenols. The most effective concentration is 30% at 24-hour incubation with an inhibition zone diameter of 3.97 mm.

Keywords : Antifungal, disc method; green betel leaf extract; inhibition Zone; Tricophyton rubrum