Antioxidant Activity Test of Sweet Potato Peel (Ipomoea batatas) from Various Regions in Indonesia and In Silico Prediction of Secondary Metabolite Compounds

Abstract

Antioxidants are compounds that can prevent oxidation reactions due to free radicals. Oxidation is a chemical reaction that can transfer electrons from a substance to an oxidizing agent. External antioxidant intake can be in the form of natural or synthetic antioxidants. The purpose of this study was to determine the antioxidant activity of sweet potato skin and to predict the groups of secondary metabolites in the sweet potato genome. So that this study can be used as a reference for further research to improve plant secondary metabolites. In this study, antioxidant activity was tested using the DPPH method using sweet potato skin samples from several regions in Indonesia, namely Riau, Tomohon, Balikpapan, Jambi, Pontianak, Kupang, Bangka, Medan, and Merauke. In the antioxidant activity test, total phenols in sweet potato skin were also tested using the Folin Ciocalteu method. Meanwhile, to predict the group of secondary metabolites using web-based bioinformatics analysis plantiSMASH. The results of the antioxidant activity test on sweet potato skin showed that the antioxidant activity of purple sweet potatoes from Pontianak was very strong at 46.17 ppm and sweet potatoes that had weak antioxidant activity were young orange sweet potatoes from Riau at 231.79 ppm. The highest total phenol content of sweet potato skin was found in white sweet potatoes from Merauke at 3.95 GAE/g and the lowest in red sweet potatoes from Jambi at 0.92 GAE/g.