eGFP Marker for Production of Type 6 and 11 Human Papilloma Virus Pseudovirus

Abstract

Human Papilloma Virus (HPV) L1 and L2 capsid proteins have a special characteristic because their ability to self-assembly into virions. This ability can be used to develop methods and products. One of them is to produce Pseudovirus (PsV) for developing Neutralization Antibody (NAb) Assay. It is usually required to evaluate immunization. In this research, this PsV have been developed to provide Pseudovirus Neutralizing Test  (PVNT). PsV HPV type 6 and 11 were regenerated using plasmid pVITRO HPV6 L1L2 and PVITRO HPV 11 L1L2, respectively. Protein self-assembly can package other genomes or non-viral DNA with them. Consequently, the addition of pCDNA 3.1 eGFP can serve as a marker for detecting the formation of PsV because of its fluorescent. Transfection of the plasmid into HEK 293 T cells were performed by using Lipofectamine. Analysis by TaliCytometer after maturation and Infection showed an increase of fluorescence that indicates the formation of PsV HPV type 6 and 11. The production of this PsV still needs more improvement to achieve productiveness for industrial purposes.