AKTIFITAS HIPOGLIKEMIK DAN ANTIOKSIDAN INFUSA DAUN AFRIKA SELATAN (VERNONIA AMYGDALINA DELILE) PADA TIKUS WISTAR (RATTUS NORVEGICUS) DIABETES

Anna Maria Dewajanti, Erma Mexcorry, Yohana B Sidabalok, Tika A H Riani

Abstract


Hyperglycemia in diabetes mellitus produces many free radicals that can increase fat peroxidation which then decomposes into malondialdehyde (a marker of cellular damage due to free radicals). The aim of the study was to determine the effect of South African leaf infusion on glucose and MDA levels. The samples were 25, grouped into 5. Each group contained 5 rats, namely the negative, positive and treatment control groups.  The treatment group was given South African leaf infusion with doses of 5%, 10% and 15% b / v. The administration of South African leaf infusion at all doses caused a decrease in glucose levels on the 15th and 30th days, where the greatest decrease was seen at a dose of 15% b / v 30 days (p <0.05). The administration of South African leaf infusion at a dose of 10% b / v and 15% b / v caused a decrease in MDA levels, where the largest decrease was also seen at doses of 15% b / v 30 days (p <0.05). This conclusion supports the notion that South African leaf infusion has hypoglycogemic activity and as an antioxidant.

Full Text:

PDF

References


Bast, A. (2001). Oxidants and Antioksidan: state of Art Am. J. Med. suppl. , 26-28

Conti, M., Morand, P.C, Levillain, P., Lemonnier. (1991). Improved fluorometric determination of malonaldehyde. Clin Chem; 37(7): 1273-5.

Danusantoso H. (2003). Peran radikal bebas terhadap beberapa penyakit paru. Jurnal Kedokteran Trisakti. 22 (1), 31-6.

Fatimah, R.N. (2015). Diabetes melitus tipe 2. Fakultas Kedokteran Universitas Journal Majority. Vol 4(5).

Grotto, D., Maria L, Valentini J, Paniz C, Schmitt G, Garcia S et al. (2009). Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantification. Química Nova. 32(1):169-74.

Ijeh, I.I, Ejike CECC (2011). Current prespective on the medicinal potentials of Vernonia amygdalina Delile. Journal of Medicinal Plants Research. 5(7).

Kementrian Kesehatas Republik Indonesia. Pusat data dan informasi. 2014.

Kharimah, N.Z, Lukmayani Y, Syafnir L. (2016). Identifikasi senyawa flavonoid pada ekstrak dan fraksi daun Afrika (Vernonia amygdalina Del.). Universitas Islam Bandung : Fakultas Matematika dan Ilmu Pengetahuan Alam.

Prasetyo, A.M. (2011). Pengaruh penambahan alpha lipoic acid terhadap perbaikan klinis penderita polineuropati diabetika [pascasarjana]. Universitas Diponegoro.

Ramatina. (2011). Effectiveness of Various antioxidant supplements on reducing oxidative status (level of plasma malondialdehide (MDA) among extension students of Bogor Agriculture University. IPB Bogor.

Retno, S.N, Sudrajat, Sudiastuti. (2016). Efektivias infusa biji jengkol (Archidendron jiringa Jack) dan daun Vernonia amygdalina Delile terhadap penurunan kadar glukosa darah mencit (Mus musculus) yang diinduksi aloksan. FMIPA Universitas Mulawarman. Sarofah, U, Sudrajat, Hariani N. (2016). Pengaruh ekstrak daun Vernonia amygdaina Delile dan Beras Ketan Hitam (Oryza sativa

glutinosa) terhadap penurunan kadar gula darah mencit (Mus musculus) yang diinduksi Aloksan. FMIPA Universitas Mulawarman.

Singh, R.P, Murthy, KNC, Jayaprakasha GK. (2002). Studies on antioxidant activity of pomegranate (punica granatum) peel and seed extract using in vitromModel. J Agri Food Chem; 50: 81-6.

Sirait, M. (2007). Penuntun fitokimia dalam farmasi. Bandung: Penerbit Institut Teknologi Bandung. Departemen Kesehatan Republik Indonesia.

Subekti, I. (2007). Tetap sehat dengan diabetes melitus. Jakarta: Balai Penerbit Fakultas Kedokteran Universitas Indonesia.

Suyoo S. (2005). Kecenderungan peningkatan jumlah penyandang diabetes, dalam penatalaksanaan diabetes terpadu. Jakarta: Balai Penerbit Fakultas Kedokteran Universitas Indonesia.

Soewoto, H., Sadikin, M., Kurniati V, Wanandi SI, Retno D, Abadi P, Prijanti AP, Harahap IP, Jusman SWA. (2001). Biokimia : eksperimen laboratorium. Jakarta : Widya Medika.

Watkins, D., Cooperstein Sj, Lazarow A. (2008). Effect of Alloxan on permeability of pancreatic islet tissue in vitro; (ditelusuri 23 Maret 2017) tersedia di: http://www.ncbi.nlm.nih.gov/pubmed/35222 13

Zamhoor, H., Pertiwi, K., Dewi RR, Nugroho G, Prangdimurti E. (2009). Pengaruh protein ransum dan pemberian the hijau terhadap kadar malondialdehida (MDA) organ hati tikus percobaan. Diunduh dari http://www.scribd.com /doc/11495362/laporan-penelitian-kadarmda


Refbacks

  • There are currently no refbacks.





Indonesian Journal of Biotechnology and Biodiversity

ISSN 2581-0014

published by:

Department of Biotechnology, Faculty of Health, Universitas Esa Unggul

Jalan Arjuna Utara No. 9, Kebon Jeruk, Jakarta Barat