Desain Primer Secara In Silico untuk Amplifikasi Gen cryIII dari Bacillus thuringiensis Isolat Lokal

Abstract

Many types of Bacillus thuringiensis isolates and subspecies are known as valuable sources for commercial important biopesticides. These Bacteria are qualify as microbiological control agents to against pests and plant disease vectors. The method can be used for cryIII gene amplification is Polymerase Chain Reaction (PCR). Primer is one important component in PCR. The aim of this research is make a primer design for cryIII gene amplification using PCR. The Primers are designed using Primary Blast tool on the National Center for Biotechnology Information (NCBI) website. The Primers that have been designed are then analyzed for their specificity with BLAST and dimer structure with DINAmelt. Analysis using the UNAfold was used to determine the secondary structure at the primer-binding site of the amplicon. Secondary structure analysis showed that no secondary structure at primer-binding site for primer. One primer that can amplify cryIII gene from B. thuringiensis was successfully designed. Further optimization of primer in the wet lab is needed to produce a good PCR reaction.