Potential of Actinomycetes to Inhibit the Biofilm Formation of Escherichia coli ATCC 25922

Abstract

Actinomycetes are soil microbes that can synthesize secondary metabolites such as antimicrobial, antifungal, and antitumor. These microbes are classified as Gram positive bacteria. They can grow very well under any conditions from fertile soils to under alkaline dry conditions. Several types of bacteria can form biofilms, which play a role in the process of infection, resistance and chronic diseases that are very difficult to treat with antimicrobial therapy. This study aims to determine the activity of Actinomycetes isolates antibiofilm on the formation of E. coli biofilms in vitro. This research was a true experimental by taking isolates from Kebun Raya Bogor with 10 points in the west area, using a 96 wells microtiter plate. Biofilm measurements on a microplate reader and obtain quantitative data in the form of Optical Density at a 595nm wavelength. The results showed that isolate no 6 had the largest percentage of 93.02% and 83.75% with an average percentage of 88.35%. (p <0.05). It can be concluded that Actinomycetes isolates from Kebun Raya Bogor have the potential to produce antibiofilms against E. coli by interfering with the quorum sensing system.