Potential of Actinomycetes Isolates as Antimicrobials for Salmonella typhi

Abstract

Actinomycetes are aerobic bacteria and Gram-positive bacilli. Acinomycets have an antimicrobial, antitumor, immunosuppressive, and antiparasitic acivity. This study aims to determine the antimicrobial potency of Actinomycetes against Salmonella typhi. The research design was a true experimental design with Actinomycetes isolates obtained from the Microbiology Laboratory of the Veteran National Development University, Jakarta. Rejuvenation of Actinomycetes isolates used spread plate method on media Starch Casein Agar (SCA) with six serial dilutions of 10^-1, 10^-2, 10^-3, 10^-4, 10^-5, and 10^-6. The antimicrobial test method used the liquid dilution method to determine the value of the minimum inhibitory concentration (MIC) with Trypticase Soy Broth (TSB) media followed by colony calculations on Salmonella Shigella Agar (SSA) media to determine the value of the minimum bactericidal concentration (MBC). The results showed that the antimicrobial activity of Actinomycetes isolates is bacteriostatic with MIC value of 10^-2.  MBC  value cannot be determined. It can be concluded that there is a potential of Actinomycetes isolates as an  antimicrobial against Salmonella typhi (p <0.05).