Study of Orchid Resistance from Cerathoriza Induction Against ORSV Infection Based on Chlorophyll Analysis

Abstract

Indonesia has a lot of orchid species. There are about 5.000 orchid species which are very popular in public. The most popular and most cultivated orchids are Phalaenopsis amabilis and Dendrobium discolor. But the presence of pests frequently hamper the orchid cultivation, one of the pest is Odontoglossum ringspot virus (ORSV). ORSV can cause orchid disease in the form of necrotic spots, which are ring-shaped spots on the leaf surface and fractions of flower color. So, inoculation of Ceratorhiza as the environmentally friendly pesticide substitute was given for the prevention. Because of that, this study should be done to know the role of Ceratorhiza in improving the resilience of orchid crops. The research was done at the Botanical Laboratory, Faculty of Mathematics and Natural Sciences, University of Lampung. The method used in this study was a completely 2 factorial randomized design. The first factor is type of orchid (Phalaenopsis amabilis and Dendrobium discolor) and the second factor is a combination of treatments (Mycorrhizal , Virus, and combination Mycorrhizae-Virus ). The observed parameter was the analysis of chlorophyll content. Data were analyzed using ANOVA at the 5% level and further testing with the Honesty Significant Difference (Tukey Test) at the 5% true level. Each treatment combination was performed 4 times for each orchid. The results showed that the inoculation of Ceratorhiza as a mycorrhiza had an influence on the chlorophyll content of Phalaenopsis amabilis and Dendrobium discolor.