Study of Orchid Resistance from Ceratorhiza sp. Induction Against ORSV Infection Based on Root Anatomy Character

Abstract

Indonesia is the country that has the largest wealth of orchid germ plasma in the world. Some popular types of orchids are Phalaenopsis amabilis and Dendrobium discolor. However, efforts to plant orchids are often hampered by viral attacks, one of which is the Odontoglossum ringpot virus (ORSV). Effective virus control on orchids can be done by using mycorrhiza, one of which is Ceratorhiza. This study aimed to determine differences in the anatomical character of the roots of Phalaenopsis amabilis and Dendrobium discolor, and efficacy of Ceratorhiza sp. in encouraging orchids to overcome ORSV infection. The design used in this study was a factorial randomized design (CRD) with 6 treatments and 4 iterations. Root anatomical observations were performed by making root cross -sections stained with phloroglucin (for lignin observation) and methylene blue staining (for peloton treatment), then observed using a microscope. The results showed that Ceratorhiza sp. in Phalaenopsis amabilis and Dendrobium discolor, the thickness of lignin in the epidermis and carrier bundles was thicker than that of treatment given only for viruses and mycorrhizal viruses.